HTLV-1 viro-immunology elucidated using thinkpeptides

Rowan et al. Retrovirology 2014 11:116   Cytotoxic T lymphocyte lysis of HTLV-1 infected cells is limited by weak HBZ protein expression, but non-specifically enhanced on induction of Tax expression

doi:10.1186/s12977-014-0116-6

Summary:

Human T-cell lymphotropic virus type 1 (HTLV-1) is a retrovirus that has been implicated in several types of human disease including aggressive T cell lymphoma. In this study Rowan et al used HTLV-1 specific CTL clones that had been expanded using peptides from thinkpeptides to gain a better understanding of the relevance of HTLV-1 HBZ and TAX proteins in immune responses to HTLV-1 infection. Both proteins are important in the virus life cycle, but TAX is clearly much more immunogenic. This suggests that HTLV-1 is under pressure to optimize the use of these proteins in its life cycle to thrive.

 

Fig 6 Rowan et al. Retrovirology 2014 11:116   doi:10.1186/s12977-014-0116-6

CTL clones expanded using peptides from thinkpeptides Tax1119-specific but not HBZ2634-specific CTLs kill Tax-expressing primary cells efficiently. Purified CD4+ cells from 7 AC (circular symbols), 1 P (square symbols), 7 HAM (triangular symbols), were cultured for 24 h alone, or in the presence of Tax-1 or HBZ-1 at an E:T ratio of 3:1. After incubation, surface and intracellular markers were stained and analysed by flow cytometry. (A) Tax staining for one representative individual; (B) frequency of Tax expression; (C) intensity of HLA-A*02:01 expression for 15 individuals in the presence or absence of CTL selection. Statistics: Wilcoxon matched pairs test, two-tailed. NS = not significant. Differences observed were statistically significant regardless of whether the outlier (HEZ) was included or excluded. Bars denote mean  SEM. See Additional file 6 for donor characteristics.

 

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