HTLV-1 viro-immunology elucidated using thinkpeptides

Rowan et al. Retrovirology 2014 11:116   Cytotoxic T lymphocyte lysis of HTLV-1 infected cells is limited by weak HBZ protein expression, but non-specifically enhanced on induction of Tax expression



Human T-cell lymphotropic virus type 1 (HTLV-1) is a retrovirus that has been implicated in several types of human disease including aggressive T cell lymphoma. In this study Rowan et al used HTLV-1 specific CTL clones that had been expanded using peptides from thinkpeptides to gain a better understanding of the relevance of HTLV-1 HBZ and TAX proteins in immune responses to HTLV-1 infection. Both proteins are important in the virus life cycle, but TAX is clearly much more immunogenic. This suggests that HTLV-1 is under pressure to optimize the use of these proteins in its life cycle to thrive.


Fig 6 Rowan et al. Retrovirology 2014 11:116   doi:10.1186/s12977-014-0116-6

CTL clones expanded using peptides from thinkpeptides Tax1119-specific but not HBZ2634-specific CTLs kill Tax-expressing primary cells efficiently. Purified CD4+ cells from 7 AC (circular symbols), 1 P (square symbols), 7 HAM (triangular symbols), were cultured for 24 h alone, or in the presence of Tax-1 or HBZ-1 at an E:T ratio of 3:1. After incubation, surface and intracellular markers were stained and analysed by flow cytometry. (A) Tax staining for one representative individual; (B) frequency of Tax expression; (C) intensity of HLA-A*02:01 expression for 15 individuals in the presence or absence of CTL selection. Statistics: Wilcoxon matched pairs test, two-tailed. NS = not significant. Differences observed were statistically significant regardless of whether the outlier (HEZ) was included or excluded. Bars denote mean  SEM. See Additional file 6 for donor characteristics.


thinkpeptides offers you large and diverse synthesis capabilities and an exceptional package of price, quality, choice, and flexibility, with timely delivery anywhere in the world.

Whether you require milligrams or grams, crude or >98% pure, 1 or thousands of peptides, we'll always offer you a great deal.

Our responsive customer service and highly qualified technical support scientists are ready to assist you with any requirements for your project.

You get a great deal from thinkpeptides!

Contact us today for a quote

Request a Quote


thinkpeptides is a brand of ProImmune

thinkpeptides, The Magdalen Centre, Oxford Science Park, Oxford, OX4 4GA, UK.

Registered in England. Company No. 387 33 86

thinkpeptides, 4281 Express Lane, Suite L2378, Sarasota, FL 34238, USA.     Privacy